STEREOLOGICAL ANALYSIS OF THE GUINEA PIG PANCREAS

Stereological Analysis of the Guinea Pig Pancreas

A stereological model which provides detailed quantitative information on the structure of the fasted, nonstimulated gland has been developed for the guinea pig pancreas. The model consists of morphologically defined space and membrane compartments which were used to describe the general composition of the tissue and the specific components of exocrine cells. The results are presented, where ap...

Studies on the Guinea Pig Pancreas

An in vitro system of guinea pig pancreatic lobules convenient for the study of secretory processes is described in this paper. In this system: (a) the over-all glandular architecture of the tissue is preserved: lobules remain morphologically intact through 5 hours; (b) amylase discharge from unstimulated lobules is low (-4%/hour) and linear over the 5 hours tested; (c) response to carbamylchol...

A Cytochemical Study on the Pancreas of the Guinea Pig

DL-leucine-1-C(14) was administered by intracardiac injection to guinea pigs and its in vivo incorporation into the proteins of various pancreatic cell fractions followed over a period of 2 hours. The pancreas was homogenized in 0.88 M sucrose and fractionated by differential centrifugation to give nuclear, zymogen, mitochondrial, microsomal, postmicrosomal, and final supernatant fractions. The...

Karyotype of Hairless Guinea pig

Chromosomal patterns of experimental animals are useful tools for cytogenetics research and animal breeding. Chromosome investigations of the hairless guinea pig are rare, therefore, karyotype of hairless guinea pigs (twelve male and female) was studied using metaphase spreads of bone marrows and G banding techniques. The chromosomes diploid number was 2n= 64 and polymorphism of three type chro...

Some observations on the feeding of the guinea pig